The Comprehensive Metabolic Phenotyping (CMP) Core provides sophisticated metabolic testing in mouse models on a fee-for-service basis.
For instance, the core offers a Sable Systems Promethion metabolic caging platform for assessing food and water consumption, locomotor activity and energy expenditure; a rodent treadmill for forced exercise studies; running wheels in the Promethion system for voluntary exercise studies; a Starr Life Sciences mouse telemetry system incorporated into the Promethion; temperature-controlled cabinets for the Promethion system to conduct circadian rhythm or adaptive thermogenesis experiments; and two Seahorse systems for metabolic experiments in cells and tissues.
The core provides a series of in vivo metabolic tests, including glucose, insulin, and pyruvate tolerance test, hyperinsulinemic-euglycemic clamps to assess insulin action, hyperglycemic clamps for measuring insulin secretion, and hypoglycemic clamps for assessing gluco-counterregulation. In addition, the core can perform arterial and venous catheterizations and hepatocyte isolations. See our equipment list for more.
The core comprises expert staff and state-of-the-art equipment to perform a cadre of metabolic tests for internal and external investigators in academia and industry. The core was established in 2016 with Patrick Fueger, Ph.D., as its founding director. The core can offer new services at the request of investigators.
Using the Comprehensive Metabolic Phenotyping Facility
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Comprehensive Metabolic Phenotyping (CMP) Core, directed by Patrick Fueger, Ph.D., helps investigators systematically characterize the metabolism and physiology of mouse models related to obesity, diabetes and cancer. The assays and services in the core range from routine metabolic testing to highly specialized assays using a sophisticated surgical catheterization model that permits access to the vasculature in freely moving, conscious animals. In addition, some assays require high-end instrumentation within the core. For the latter, the core offers a 16-unit Sable Systems Promethion metabolic caging platform that contains voluntary running wheels and an EchoMRI 3-in-1 system for body and tissue composition analysis. In addition, the core has all of the surgical equipment, Harvard Apparatus infusion pumps, Alpha Trak glucose monitors, and peripheral equipment for assessing insulin release in vivo with the hyperglycemic clamp technique, which is considered the platinum standard for evaluating islet function, and quantifying insulin action in vivo using the gold standard hyperinsulinemic-euglycemic clamp technique. Routine metabolic testing includes glucose, insulin and pyruvate tolerance tests and blood sampling to measure circulating hormones (e.g., insulin, C-peptide and glucagon) with ELISAs and RIAs and various metabolites (e.g., cholesterol and free fatty acids) with enzymatic assays. Finally, the core offers scientific consultation, total project management and first-pass data analysis.