City of Hope’s Analytical Cytometry Core (ACC) Facility provides researchers with high-quality flow cytometry instrumentation, as well as expertise in analyzing and/or sorting sample populations of interest via interpretation of their physical, fluorescent and/or light-scattering properties. The facilities and their services are available to both City of Hope and non-City of Hope researchers.
The Analytical Cytometry Core has 7 cell sorters (ARIA III, 4-ARIA Fusion, and ARIA II SORP, and Tyto Cell Sorter) and 16 analyzers (LSRII, Symphony, 3-Fortessa, 3-Fortessa X-20, 3-Attune, C6, Novocyte 3005, 2-Quanteon 4025, and Aurora). Analyzers and sorters are available for unassisted end user runs following completion of appropriate training. Additionally, ACC has a Nanosight NS300 for submicron particle characterization. Lastly, a Helios Mass Cytometer is available with core assisted operation.
As an added convenience, the ACC supports several off-line workstations where investigators can analyze their ACC-derived data. ACC software includes Flowjo, FCS Express, DiVa, Attune Software and Spectroflo.
Alexander Spalla, B.S.
Senior Research Associate
Ni Feng, B.S.
Research Associate II
Shaun Hsueh, B.S.
Research Associate II
David Rose, Ph.D.
Staff Scientist
Ian Buczko, B.S.
Research Associate II
Sumana Majumdar, M.S.
Research Associate II
The Analytical Cytometry Core uses flow cytometric principles to analyze and sort samples (cells or particles) based on fluorescence or physical properties.
- Cell sorting
- Flow cytometry analysis
- Data analysis
- Equipment training — analyzer
- Equipment training — sorter
- FCS Express and Flowjo software access
- Software training
- Technical assistance
The Analytical Cytometry Core (ACC) comprises seven cell sorters, 14 analytical cytometers, CyTOF-Helios mass cytometer, and a nanoparticle analysis instrument.
Our cell sorters
- ARIA III (Becton Dickinson) with four lasers (405nm, 488nm, 561nm and 633nm) and up to 15 fluorescent parameters. This sorter is contained in a biosafety cabinet.
- ARIA II SORP (Becton Dickinson) with six lasers (355nm, 405nm, 457nm, 488nm, 561nm and 640nm) and up to 18 simultaneous fluorescent parameters. This sorter is contained in a biosafety cabinet.
- ARIA Fusion (Becton Dickinson) with five lasers (355nm, 405nm, 488nm, 561nm and 640nm) and up to 18 simultaneous fluorescent parameters. These sorters are contained in a biosafety cabinet.
- Tyto Cell Sorter with 3 lasers (405nm, 488nm, and 638nm) and 8 fluorescent detectors. Requires specific cartridges for operation.
Our analytical cytometers
- Novocyte3005 with 3 laser (405nm, 488nm, 640nm) system equipped with 14 fluorescent detectors.
- Quanteon 4025 with 4 laser system (405nm, 488nm, 561nm, 637nm) equipped with 25 fluorescent detectors.
- Aurora with 5 laser system (355nm, 405nm, 488nm, 561nm, 640nm) full spectrum analyzer equipped with 64 fluorescent detectors.
- Fortessa SORP (Becton Dickinson) with five lasers (355nm, 405nm, 488nm, 561nm and 640nm) and up to 18 simultaneous fluorescent parameters. Also available is an HTS module for 96-well sample delivery allowing for automation.
- Fortessa X-20 (Becton Dickinson) with five lasers (355nm, 405nm, 488nm, 561nm and 640nm) and up to 18 simultaneous fluorescent parameters. Also available is an HTS module for 96-well sample delivery allowing for automation.
- LSRII (Becton Dickinson) with five lasers (355nm, 405nm, 488nm, 561nm and 640nm) and up to 15 simultaneous fluorescent parameters.
- FACSymphony (Becton Dickinson) with five high-powered lasers (355nm, 405nm, 488nm, 561nm and 640nm) and up to 28 simultaneous fluorescent parameters.
- C6 (Becton Dickinson) with four fluorescent detectors and two lasers (488nm-3 detectors and 640nm-1 detector).
- Attune cytometer (ThermoFisher) with four lasers (405nm, 488nm, 561nm and 640nm) and 14 simultaneous fluorescent parameters. It has sample delivery from tube or 96-well format integrated in the system (controlled by software).
Other instrumentation
- NanoSight NS300 (Malvern) allows for the characterization of submicron size particles.
- CyTOF-Helios (Fluidigm) utilizes metal-tagged antibodies or reagents to detect up to 50 simultaneous parameters on a per cell basis.
ACC also provides training for user operation of the instruments, basic flow theory, software, and data analysis for result generation and presentation. Other functions include application assistance, experimental design, and site license management for Flowjo and FCSexpress software.
Current pricing can be found on iLab Solutions.
The Aria Fusion cell sorters (Becton Dickinson) are five-laser (355nm, 405nm, 488nm, 561nm and 640nm) with 18 simultaneous fluorescent detectors.
The Aria II SORP (Becton Dickinson) is a six-laser system (355nm, 405nm, 457nm, 488nm, 561nm and 640nm) with a maximum of 17 simultaneous fluorescent detectors.
The Aria III cell sorter (Becton Dickinson) is a four-laser system (405nm, 488nm, 561nm and 633nm) with a maximum of 15 fluorescent detectors.
The Attune NxT (ThermoFisher) is a four-laser (405nm, 488nm, 561nm and 640nm) 14 fluorescent detector instrument.
The Aurora is a 5 laser (355nm, 405nm, 488nm, 561nm, 640nm) full spectrum analyzer equipped with 64 fluorescent detectors. Full spectrum cytometers utilize the entire emission spectra of fluorophores which allows for greater data resolution and the utilization of more fluorophores simultaneously when compared with traditional flow cytometers. This system has the option of utilizing either 12x75mm 5mL FACS tubes or from 96-well plate formats f or data acquisition. This instrument is available for trained users to operate, or user samples can be analyzed by the core staff at an additional cost.
The C6 (Becton Dickinson) is a two-laser (488nm and 640nm) system. Up to three fluorescent detectors are available for the 488nm laser. One detector is available for the 640nm laser. Trained users may operate this cytometer.
CyTOF-Helios (Fluidigm) utilizes mass cytometry, which uniquely combines time-of-flight mass spectrometry with metal-tagged antibodies or reagents to detect up to 50 simultaneous parameters on a per cell basis.
This Becton Dickinson cytometer has five lasers (355nm, 405nm, 488nm, 561nm and 640nm).
These instruments are five-laser analytical cytometers. The Becton Dickinson cytometers are special order research products equipped with 355nm (UV), 405nm, 488nm, 561nm and 640nm lasers.
The Miltenyi Tyto cell sorter is a microfluidics based system. It contains 3 lasers (405nm, 488nm, and 638nm) and 8 fluorescent detectors. Requires specific cartridges for operation.
NanoSight NS300 (Malvern) allows for the characterization of submicron-sized particles.
The Novocyte is a 3 laser (405nm, 488nm, 640nm) system equipped with 14 fluorescent detectors. Capable of running samples from either 12x75mm 5mL tubes or from 96-well plate formats. This instruments is available for trained users to operate, or user samples can be analyzed by the core staff at an additional cost.
The Quanteon 4025 is a 4 laser system (405nm, 488nm, 561nm, 637nm) equipped with 25 fluorescent detectors. Capable of running samples from 12x75mm 5mL tubes, microfuge tubes, and from 96-well plate formats. These instruments are available for trained users to operate, or user samples can be analyzed by the core staff at an additional cost.
In a given year, City of Hope conducts more than 400 clinical trials enrolling more than 6,000 patients.
City of Hope is focused on basic and clinical research in cancer, diabetes and other life-threatening diseases.
