The Chen laboratory is interested in discovering novel genetic/epigenetic regulations and the associated molecular mechanisms of both protein-coding genes and noncoding RNAs (e.g., microRNAs) in normal developmental processes (e.g., hematopoiesis) and tumorigenesis (e.g., leukemogenesis). In recent years, Chen and colleagues have been focusing on both basic and translational research associated with RNA/DNA epigenetics, especially RNA methylation/demethylation related to the N6 methyladenosine (m6A) machinery and DNA demethylation related to the TET1/2/3 family, and have developed several small-molecule compound inhibitors to selectively target cancer-related RNA/DNA epigenetic modifiers that hold therapeutic potential. The ultimate goal of the lab is to translate the laboratory discoveries into the development of effective novel therapeutic strategies to treat cancers, especially acute leukemia, in the clinic.
Professor and Chair of the Department of Systems Biology, Jianjun Chen conducts research that focuses on systems biology. He received his Ph.D. from Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai, China, and then conducted postdoctorate training with Dr. Janet D. Rowley at University of Chicago, Department of Medicine where he launched his independent laboratory in 2009.
2020-present, Ph.D. candidate, Hematology, Fujian Medical University, Fuzhou, Fujian, China
2014, B.S. and M.D., Clinical Medicine, Fujian Medical University, Fuzhou, Fujian, China
2021-present, Visiting Scholar, Department of Systems Biology, Beckman Research Institute of City of Hope, Monrovia, CA
2018-present, Attending Physician, Department of Hematology, Fujian Medical University Union Hospital, Fuzhou, Fujian, China
2014, Outstanding Graduate Student of Fujian Medical University
2012, National Scholarship for Graduate Students
2020-present, Secretary of the Youth Committee of the Hematology Physician of Fujian Medical Association
The effect of interfering TGF-β receptor ǁ expression on NB4 cells proliferation and different response to ATRA and ATO. Chin J Pathol. 2018, 34(10): 1729-1735.
Chen M, Zhang S, Chen Y, Guo Y, Wu Y.
Expression and role of granulocyte macrophage colony-stimulating factor receptor (GM-CSFR) and granulocyte colony-stimulating factor receptor(G-CSFR) on Ph-positive acute B lymphoblastic leukemia. Hematology. 2018, 23(8): 439-447.
Tan M, Chen M, Chen Y, Wu Y.
Ying Qing received her M.D. from Shandong University, China, and her Ph.D. from the Irell & Manella Graduate School of Biological Sciences at City of Hope. She joined the Jianjun Chen lab in 2017. Her current research is focused on (1) deciphering the common epitranscriptomic mechanism underlying obesity and cancer and (2) comparing the different roles of TET1 protein in leukemia and solid tumors and identifying the different regulatory pathways. Her long-term research interests involve developing a comprehensive understanding of the mechanisms, especially epigenetic or epitranscriptomic mechanism, underlying cancer initiation, promotion, and progression, and how to develop novel effective anti-cancer therapies based on that knowledge.
Uncovering the common epitranscriptomic signaling pathway that mediates the development of obesity and cancer induced by Western lifestyle
Different roles of TET1 in leukemia and solid tumors
2021, Ph.D., Biological Sciences, Irell & Manella Graduate School of Biological Sciences at City of Hope, Duarte, CA
2015, M.D., Clinical Medicine (Internal Medicine), Shandong University, Jinan, China
2021-present, Postdoctoral Fellow, Department of Systems Biology, Beckman Research Institute of City of Hope, Duarte, CA
Qing, Y.*, Dong, L.*, Gao, L.*, Li, C., Li, Y., Han, L., Prince, E., Tan, B., Deng, X., Wetzel, C., Shen, C., Gao, M., Chen, Z., Li, W., Zhang, B., Braas, D., Hoeve, J.T., Sanchez, G.J., Chen, H., Chan, L.N., Chen, C.W., Ann, D., Jiang, L., Müschen, M., Marcucci, G., Plas, D., Li, Z., Su, R., & Chen, J. R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis. Molecular Cell. 2021;81(5):922-39. doi: 10.1016/j.molcel.2020.12.026, 2021. PubMed PMID: 33434505. (*Co-first authors)
Qing, Y., Su, R., & Chen, J. RNA modifications in hematopoietic malignancies: A new research frontier. Blood. 2021;138(8):637-648. doi: 10.1182/blood.2019004263. PubMed PMID: 34157073.
Qing, Y., Gao, L., Han, L., Su, R., & Chen, J. Evaluation of glycolytic rates in human hematopoietic stem/progenitor cells after target gene depletion. STAR Protoc. 2021;2(2):100603. doi: 10.1016/j.xpro.2021.100603. PubMed PMID: 34169295.
Su, R.*, Qing, Y.*, & Chen, J. Targeting Differentiation Blockade in AML: New Hope from Cell Surface-based CRISPR Screens. Cell Stem Cell. 2021;28(4):585-7. doi: 10.1016/j.stem.2021.03.006. PubMed PMID: 33798415. (*Co-first authors)
Qing, Y., Tian, Z., Bi, Y., Wang, Y., Long, J., Song, C.X., & Diao, J. Quantitation and mapping of the epigenetic marker 5-hydroxymethylcytosine. Bioessays. 2017;39(5):1700010. doi: 10.1002/bies.201700010. Review. PubMed PMID: 28332209.
Qing, Y., Wang, M., Lin, Y.M., Wu, D., Zhu, J.Y., Gao, L., Liu, Y.Y., & Yin, T.F. Correlation between Helicobacter pylori-associated gastric diseases and colorectal neoplasia. World J Gastroenterol. 2016;22(18):4576-84. doi: 10.3748/wjg.v22.i18.4576. PubMed PMID: 27182167.
Association between metabolic disorder and cancer development
2013-2018, Ph.D., Shandong University, Jinan, China
2016-2018, visiting Ph.D. student, University of Cincinnati, OH
2008-2013, M.D., Shandong University, Jinan, China
2020-2022, Fellowship in Pancreatic Surgery, Qilu Hospital of Shandong University, Jinan, China
2018-2020, Residency in General Surgery, Qilu Hospital of Shandong University, Jinan, China
2022-present, Postdoctoral fellow, Department of Systems Biology, Beckman Research Institute of City of Hope, Duarte, CA
2019, First prize of Shandong Province Science and Technology Progress Award (11/12)
2014, Outstanding Graduate Students of Shandong University
2012, National Scholarship
2009, National Endeavor Scholarship
Wu, D., Cheng, Y.G., Huang, X., Zhong, M.W., Liu, S.Z., & Hu, S.Y. Downregulation of lncRNA MALAT1 contributes to renal functional improvement after duodenal-jejunal bypass in a diabetic rat model. J Physiol Biochem. 2018 Aug;74(3):431-439. doi: 10.1007/s13105-018-0636-y. Epub 2018 May 21. PMID: 29781038.
Wu, D.*, Yan, Z.B.*, Cheng, Y.G., Zhong, M.W., Liu, S.Z., Zhang, G.Y., & Hu, S.Y. Deactivation of the NLRP3 inflammasome in infiltrating macrophages by duodenal-jejunal bypass surgery mediates improvement of beta cell function in type 2 diabetes. Metabolism. 2018 Apr;81:1-12. doi: 10.1016/j.metabol.2017.10.015. Epub 2017 Nov 10. PMID: 29129820.
Zhan, H.X., Xu, J.W., Wu, D., Wu, Z.Y., Wang, L., Hu, S.Y., & Zhang, G.Y. Neoadjuvant therapy in pancreatic cancer: a systematic review and meta-analysis of prospective studies. Cancer Med. 2017 Jun;6(6):1201-1219. doi: 10.1002/cam4.1071. Epub 2017 May 23. PMID: 28544758; PMCID: PMC5463082.
Identification and analysis DNA methylation in leukemia
Screening and optimization of new targets for leukemia immunotherapeutic
2007, M.D., Genetics, Henan University, China
2014, Ph.D., Genetics, Southeast University, China
2021-present, Visiting Scholar, Department of Systems Biology, Beckman Research Institute of City of Hope, Duarte, CA
2014 -present, Resident, Central laboratory, Huai’an First People’s Hospital, Jiangsu, China
2021, Huai’an Science and Technology Star
2021-present, Medical Genetics of Jiangsu Province
Han, X., Zhang, C.*, & Ding, L.S.* PTIP Inhibits Cell Invasion in Esophageal Squamous Cell Carcinoma via Modulation of EphA2 Expression. Frontiers in oncology. 2021 Mar 23; 11: 629916.
Zhang, C., et al. KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia. Theranostics. 2021 Jan; 11(6):2691-2705.
Zhang, C., et al. Drosophila UTX coordinates with p53 to regulate ku80 expression in response to DNA damage. PLoS One. 2013 Nov 12; 8(11):e78652.
FTO Plays an Oncogenic Role in Acute Myeloid Leukemia as a N6-Methyladenosine RNA Demethylase
Li Z, Weng H, Su R, Weng X, Zuo Z, Li C, Huang H, Nachtergaele S, Dong L, Hu C, Qin X, Tang L, Wang Y, Hong GM, Huang H, Wang X, Chen P, Gurbuxani S, Arnovitz S, Li Y, Li S, Strong J, Neilly MB, Larson RA, Jiang X, Zhang P, Jin J*, He C*, Chen J*. Cancer Cell. 2017 Jan 9;31(1):127-141. PMCID: PMC5234852. **Cover Story of this issue of Cancer Cell ***Highlighted (Research Watch) by Cancer Discovery (2017 Feb;7(2):OF9)
R-2HG exhibits anti-tumor activity by targeting FTO/m6A/MYC/CEBPA signaling
Su R, Dong L, Li C, Nachtergaele S, Wunderlich M, Qing Y, Deng X, Wang Y, Weng X, Hu C, Yu M, Skibbe J, Dai Q, Zou D, Wu T, Yu K, Weng H, Huang H, Ferchen K, Qin X, Zhang B, Qi J, Sasaki AT, Plas D, Bradner JE, Wei M, Marcucci G, Jiang X, Mulloy J, Jin J*, He C*, Chen J*. Cell. 2018 Jan 11;172(1-2):90-105. PMCID: PMC5766423. **Highlighted (Research Watch) by Cancer Discovery (2018 Feb;8(2):137)
METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and Promotes Leukemogenesis via mRNA m6A Modification
Weng H, Huang H, Wu H, Qin X, Zhao BS, Dong L, Shi H, Skibbe J, Shen C, Hu C, Sheng Y, Wang Y, Wunderlich M, Zhang B, Dore L, Su R, Deng X, Ferchen K, Li C, Sun M, Lu Z, Jiang X, Marcucci G, Mulloy J, Yang J, Qian Z*, Wei M*, He C*, Chen J*. Cell Stem Cell. 2018 Feb 1;22(2):191-205. PMCID: PMC5860916. **Highlighted by Cell Stem Cell with a Preview article (2018; 22:139–141); ***Best of Cell Stem Cell 2018 collection (one of the seven best articles published by Cell Stem Cell in 2018)
Recognition of RNA N6-methyadenosine by IGF2BP proteins Enhances mRNA Stability and Translation
Huang H, Weng H, Sun W, Qin X, Shi H, Wu H, Zhao BS, Mesquita A, Liu C, Yuan CL, Hu YC, Hüttelmaier S, Skibbe J, Su R, Deng X, Dong L, Sun M, Li C, Nachtergaele S, Wang Y, Hu C, Ferchen K, Greis KD, Jiang X, Wei M, Qu L, Guan JL, He C*, Yang J*, Chen J*. Nature Cell Biology. 2018 Mar;20(3):285-295. PMCID: PMC5826585. **Featured by Nature Cell Biology with a News & Views article (2018; 20:230–232)
RNA N6-methyladenosine modification in Cancers: Current Status and Perspective
Deng X, Su R, Weng H, Huang H, Li Z, Chen J*.Cell Research. 2018 May; 28(5):507-517. PMCID: PMC5951805.
Huang H, Weng H, Zhou K, Wu T, Zhao BS, Sun M, Chen Z, Deng X, Xiao G, Auer F, Klemm L, Wu H, Zuo Z, Qin X, Dong Y, Zhou Y, Qin H, Tao S, Du J, Liu J, Lu Z, Yin H, Mesquita A, Yuan CL, Hu YC, Sun W, Su R, Dong L, Shen C, Li C, Qing Y, Jiang X, Wu X, Sun M, Guan JL, Qu LH, Wei M, Müschen M, Huang G*, He C*, Yang JH*, Chen J*. Nature. 2019 Mar 21;567(7748):414-419. PMCID: PMC6438714. **Featured by Nature Reviews Genetics (2019 May; 20(5):254-255), Nature Chemical Biology (2019 May; 15(5):427), and Cancer Research (2019 May, 79: 2445-2446).
Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
Huang Y, Su R, Sheng Y, Dong L, Dong Z, Xu H, Ni T, Zhang ZS, Zhang T, Li C, Han L, Zhu Z, Lian F, Wei J, Deng Q, Wang Y, Wunderlich M, Gao Z, Pan G, Zhong D, Zhou H, Zhang N, Gan J, Jiang H, Mulloy JC, Qian Z*, Chen J*, Yang CG*. Cancer Cell. 2019 Apr 15;35(4):677-691. PMCID: PMC6812656. **Highlighted by Cancer Cellwith a Preview article (2019 Apr 15;35(4):540-541).
RNA Modifications in Cancer: Functions, Mechanisms and Therapeutic Implications.
RNA demethylase ALKBH5 selectively promotes tumorigenesis and cancer stem cell self-renewal in acute myeloid leukemia
Shen C, Sheng Y, Zhu A, Robinson S, Jiang X, Dong L, Chen H, Su R, Yin Z, Li W, Deng X, Chen Y, Hu YC, Weng H, Huang H, Prince E, Cogle CR, Sun M, Zhang B, Chen CW, Marcucci G, He C*, Qian Z*, Chen J*. Cell Stem Cell. 2020 Jul 2;27(1):64-80. PMCID: PMC7335338. **Highlighted by Cell Stem Cell with a Preview article (2020 Jul 2;27(1):3-5).
Targeting FTO suppresses cancer stem cell maintenance and immune evasion
Su R, Dong L, Li Y, Gao M, Han L, Wunderlich M, Deng X, Li H, Huang Y, Gao L, Li C, Zhao Z, Robison S, Tan B, Qing Y, Qin X, Prince E, Xie J, Qin H, Li W, Shen C, Sun J, Prakash K, Weng H, Huang H, Chen Z, Zhang B, Wu X, Olsen MJ, Muschen M, Marcucci G, Ravi S, Li, L, Fathi AT, Li Z, Mulloy JC, Wei M, Horne D, Chen J*. Cancer Cell. 2020 Jul 13;38(1):79-96. PMCID: PMC7363590. **Highlighted by Molecule Cell with a Spotlight article (2020 Jun 18;78(6):996-998); ***Highlighted by Nature Reviews Drug Discovery (2020 Aug;19(8):510).
R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis
Qing Y, Dong L, Gao L, Li C, Li Y, Han L, Prince E, Tan B, Deng X, Wetzel C, Shen C, Gao M, Chen Z, Li W, Zhang B, Braas D, Hoeve Jt, Sanchez GJ, Chen H, Chan LN, Chen CW, Ann D, Jiang L, Müschen M, Marcucci G, Plas DP, Li Z, Su R*, Chen J*. Molecular Cell. 2021 Mar 4;81(5):922-939. PMCID: PMC7935770. **Highlighted by Blood Science (3(2):62-63, April 2021).
Targeting differentiation blockade in AML: New hope from cell surface-based CRISPR screens
Su R, Qing Y, Chen J*. Cell Stem Cell. 2021 Apr 1;28(4):585-587.
RNA modifications in hematopoietic malignancies: A new research frontier
Qing Y, Su R, Chen J*. Blood. 2021 Jun 22;138(8):637-48. PMCID: PMC8394902.
DNA N(6)-methyldeoxyadenosine in mammals and human diseases
Shen C, Wang K, Deng X, Chen J*.Trends Genet. 2022 Jan 3;S0168-9525(21)00353-X. doi: 10.1016/j.tig.2021.12.003. Online ahead of print.
METTL16 exerts an m6A-independent function to facilitate translation and tumorigenesis
Su R*, Dong L, Gao M, Li Y, He PC, Liu W, Wei J, Gao L, Han L, Deng X, Zhao Z, Prince E, Tan B, Qing Y, Qin X, Shen C, Zhou K, Chen Z, Xue J, Li W, Qin H, Wu X, Sun M, Nam Y, Chen CW, Horne D, Rosen ST, He C*, Chen J*. Nature Cell Biology. 2022 Feb;24(2):205-216. PMCID: PMC9070413.
FTO in cancer: Functions, molecular mechanisms and therapeutic implications
Li Y, Su R, Deng X, Chen Y, Chen J*. Trends in Cancer. 2022 Mar 25:S2405-8033(22)00054-1. doi: 10.1016/j.trecan.2022.02.010. Online ahead of print.
m6A RNA modifications are measured at single-base resolution across the mammalian transcriptome
Hu L*, Liu S, Peng Y, Ge R, Su R, Senevirathne C, Harada BT, Dai Q, Wei J, Zhang L, Hao Z, Luo L, Wang H, Wang Y, Luo M, Chen M*, Chen J*, He C*. Nature Biotechnology. 2022 Mar 14. doi: 10.1038/s41587-022-01243-z. Online ahead of print. PMID: 35288668
RNA-binding Proteins in Regulating mRNA Stability and Translation: Roles and Mechanisms in Cancer
Li W, Deng X, Chen J*. Seminars in Cancer Biology. 2022 Apr 2:S1044-579X(22)00082-7. doi: 10.1016/j.semcancer.2022.03.025. Online ahead of print.
RNA N6-methyladenosine reader YTHDC1 is essential for TGF-beta-mediated metastasis of triple negative breast cancer
Tan B, Zhou K, Liu W, Prince E, Qing Y, Li Y, Han L, Qin X, Su R, Pokharel SP, Yang L, Zhao Z, Shen C, Li W, Chen Z, Zhang Z, Deng X, Small A, Wang K, Leung K, Chen CW, Shen B, Chen J*. Theranostics. 2022. In Press.